In the spirit of googling oneself, I discovered that "digsy" is the name of an actual British rockstar and Noel Gallagher from Oasis wrote a song for him in their first album Definately Maybe called "Digsy's Dinner." I know that's not my name, but I was still a little taken aback...
I have a secret desire to write for the daily cal blog "The Clog." It says that I need a funny voice. My voice is sort of funny, if not annoying. Stephie said that I have a funny laugh and then wondered whether my future significant other would laugh like me.
I just checked whether the clog link was to the right site. It most definately is not. Maybe I should write for them instead.
Thursday, September 20, 2007
Wednesday, September 12, 2007
Donna Almendrala
MCB 110L
September 12, 2007 - Experiment 7
Procedure I:
Goal – Digest the pET28-lacZ plasmid preps from lab 5 and then size the fragments on a gel.
1. For the pET28-lacZ, do 5 restriction digests using the following:
|
| Water (uL) | Buffer (uL) | DNA (uL) | Enzyme (uL) |
| Cla I |
| 4: 2 + BSA | 5 | 2 |
| Sac I |
| 1: 2 + BSA | 5 | 0.5 |
| Ssp I |
| 2: 2 | 5 | 2 |
| Hind III/EcoR V |
| 2: 2 + BSA | 5 | 0.5 + 0.5 |
| Ssp I/EcoR V |
| 2: 2 + BSA | 5 | 2 + 0.5 |
2. Digest for about 1-2 hrs in 37˚C water bath.
3. Run the digests on a 1% agarose gel at 100V for an hour. Make sure to add a 1kb DNA standard lane. You can share a gel with another group.
4. Use 4uL of dye and 20uL of each digest for the lane.
5. After gel is run, photograph it and estimate the sizes of the DNA fragments.
6. Draw a restriction map for pET28- and deduce the orientation of the lacZ gene insert.
Expected Result – The gel should follow the same pattern as one of the mock gels shown on the previous page. Based on which mock gel it resembles, the orientation can be ascertained as either clockwise or counterclockwise.
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