It's too late to apologize. Too late!!!!!!!

After dinner on Christmas, Chris picked me up and we went for an adventure down in Livermore. While having grown up in the Tri-Valley, Livermore remains uncharted territory for the both of us. I only went there for two things, Costco and Las Po. There's a place called Donut Wheel there on First Street and it was supposedly open 24 hours. Since everything is closed on Christmas, besides the movies, he thought it would be fun to see how hardcore Donut Wheel was and if it stayed open for the holiday, even though neither of us particularly wanted a donut at 11pm. We used our natural intuition and his iPhone to find the place and it turns out that Livermore has a really nice downtown area. The donuts were .75, and pretty damn awesome. I think we should try to go again and catch the donuts when they come out hot from the oven. I was mesmerized by the giant tray of donut holes, but I was too full from everything else so I'm just left to build it up in my mind as one of the things I simply must have.

Lost in fog and love and faithless fear

I came home for Thanksgiving break yesterday. It's been nice, my little brother has taken to calling me "Bud-o" rather than the usual "buddy." It's adorable and endearing; he makes me feel purely and innocently loved. He has no idea of course, but how he blindly looks up to me, gives me something so cling to when it feels like I have no clue who I am anymore.

song of the moment:
Citrus - The Hold Steady

Thunder!

this is a test.

It comes back to you

Rachel called me today from all the way across the world. It was amazing, she showed me how to add contacts to Skype and then she even gave me the names of some of her friends so that I could have friends on my Skype list as well.

Today, I am going to wear my Cal Poly shirt to celebrate California public education.

Oh, what a wonderful world!

Digsy's Diner

In the spirit of googling oneself, I discovered that "digsy" is the name of an actual British rockstar and Noel Gallagher from Oasis wrote a song for him in their first album Definately Maybe called "Digsy's Dinner." I know that's not my name, but I was still a little taken aback...

I have a secret desire to write for the daily cal blog "The Clog." It says that I need a funny voice. My voice is sort of funny, if not annoying. Stephie said that I have a funny laugh and then wondered whether my future significant other would laugh like me.

I just checked whether the clog link was to the right site. It most definately is not. Maybe I should write for them instead.

Oh sh!$

What can't Google do these days?

Donna Almendrala

MCB 110L

September 12, 2007 - Experiment 7

Procedure I:

Goal – Digest the pET28-lacZ plasmid preps from lab 5 and then size the fragments on a gel.

1. For the pET28-lacZ, do 5 restriction digests using the following:

	Water (uL)	Buffer (uL)	DNA (uL)	Enzyme (uL)
Cla I		4: 2 + BSA	5	2
Sac I		1: 2 + BSA	5	0.5
Ssp I		2: 2	5	2
Hind III/EcoR V		2: 2 + BSA	5	0.5 + 0.5
Ssp I/EcoR V		2: 2 + BSA	5	2 + 0.5

2. Digest for about 1-2 hrs in 37˚C water bath.

3. Run the digests on a 1% agarose gel at 100V for an hour. Make sure to add a 1kb DNA standard lane. You can share a gel with another group.

4. Use 4uL of dye and 20uL of each digest for the lane.

5. After gel is run, photograph it and estimate the sizes of the DNA fragments.

6. Draw a restriction map for pET28- and deduce the orientation of the lacZ gene insert.

Expected Result – The gel should follow the same pattern as one of the mock gels shown on the previous page. Based on which mock gel it resembles, the orientation can be ascertained as either clockwise or counterclockwise.